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, Irene Cadenas-Jiménez Microbiology Department, Hospital Universitari Bellvitge, IDIBELL-UB , Feixa Llarga s/n, 08907 L’Hospitalet de Llobregat, Barcelona , Spain Research Network for Respiratory Diseases (CIBERES), ISCIII , Madrid , Spain Search for other works by this author on: Oxford Academic Lucía Saiz-Escobedo Microbiology Department, Hospital Universitari Bellvitge, IDIBELL-UB , Feixa Llarga s/n, 08907 L’Hospitalet de Llobregat, Barcelona , Spain Search for other works by this author on: Oxford Academic Anna Carrera-Salinas Microbiology Department, Hospital Universitari Bellvitge, IDIBELL-UB , Feixa Llarga s/n, 08907 L’Hospitalet de Llobregat, Barcelona , Spain Search for other works by this author on: Oxford Academic Xenia Camprubí-Márquez Microbiology Department, Hospital Universitari Bellvitge, IDIBELL-UB , Feixa Llarga s/n, 08907 L’Hospitalet de Llobregat, Barcelona , Spain Search for other works by this author on: Oxford Academic Sara Calvo-Silveria Microbiology Department, Hospital Universitari Bellvitge, IDIBELL-UB , Feixa Llarga s/n, 08907 L’Hospitalet de Llobregat, Barcelona , Spain Research Network for Respiratory Diseases (CIBERES), ISCIII , Madrid , Spain Search for other works by this author on: Oxford Academic Paula Camps-Massa Microbiology Department, Hospital Universitari Bellvitge, IDIBELL-UB , Feixa Llarga s/n, 08907 L’Hospitalet de Llobregat, Barcelona , Spain Respiratory Medicine Department, Hospital Universitari Bellvitge, IDIBELL-UB , Barcelona , Spain Search for other works by this author on: Oxford Academic Dàmaris Berbel Microbiology Department, Hospital Universitari Bellvitge, IDIBELL-UB , Feixa Llarga s/n, 08907 L’Hospitalet de Llobregat, Barcelona , Spain Research Network for Respiratory Diseases (CIBERES), ISCIII , Madrid , Spain Search for other works by this author on: Oxford Academic Fe Tubau Microbiology Department, Hospital Universitari Bellvitge, IDIBELL-UB , Feixa Llarga s/n, 08907 L’Hospitalet de Llobregat, Barcelona , Spain Research Network for Respiratory Diseases (CIBERES), ISCIII , Madrid , Spain Search for other works by this author on: Oxford Academic Salud Santos Research Network for Respiratory Diseases (CIBERES), ISCIII , Madrid , Spain Respiratory Medicine Department, Hospital Universitari Bellvitge, IDIBELL-UB , Barcelona , Spain Search for other works by this author on: Oxford Academic M Angeles Domínguez Microbiology Department, Hospital Universitari Bellvitge, IDIBELL-UB , Feixa Llarga s/n, 08907 L’Hospitalet de Llobregat, Barcelona , Spain Research Network for Infectious Diseases, CIBERINFEC, ISCIII , Madrid , Spain Search for other works by this author on: Oxford Academic
https://orcid.org/0000-0002-3689-3360 , Aida González-Díaz Microbiology Department, Hospital Universitari Bellvitge, IDIBELL-UB , Feixa Llarga s/n, 08907 L’Hospitalet de Llobregat, Barcelona , Spain Research Network for Respiratory Diseases (CIBERES), ISCIII , Madrid , Spain Search for other works by this author on: Oxford Academic Carmen Ardanuy Microbiology Department, Hospital Universitari Bellvitge, IDIBELL-UB , Feixa Llarga s/n, 08907 L’Hospitalet de Llobregat, Barcelona , Spain Research Network for Respiratory Diseases (CIBERES), ISCIII , Madrid , Spain Search for other works by this author on: Oxford Academic Sara Martí Microbiology Department, Hospital Universitari Bellvitge, IDIBELL-UB , Feixa Llarga s/n, 08907 L’Hospitalet de Llobregat, Barcelona , Spain Research Network for Respiratory Diseases (CIBERES), ISCIII , Madrid , Spain Corresponding author. E-mail: smartinm@bellvitgehospital.cat Search for other works by this author on: Oxford Academic
Journal of Antimicrobial Chemotherapy, dkae214, https://doi.org/10.1093/jac/dkae214
Published:
01 July 2024
Article history
Received:
15 March 2024
Accepted:
07 June 2024
Published:
01 July 2024
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Irene Cadenas-Jiménez, Lucía Saiz-Escobedo, Anna Carrera-Salinas, Xenia Camprubí-Márquez, Sara Calvo-Silveria, Paula Camps-Massa, Dàmaris Berbel, Fe Tubau, Salud Santos, M Angeles Domínguez, Aida González-Díaz, Carmen Ardanuy, Sara Martí, Molecular characterization of macrolide resistance in Haemophilus influenzae and Haemophilus parainfluenzae strains (2018–21), Journal of Antimicrobial Chemotherapy, 2024;, dkae214, https://doi.org/10.1093/jac/dkae214
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Abstract
Objectives
This study aimed to explore the prevalence of macrolide resistance and the underlying resistance mechanisms in Haemophilus influenzae (n = 2556) and Haemophilus parainfluenzae (n = 510) collected between 2018 and 2021 from Bellvitge University Hospital, Spain.
Methods
Antimicrobial susceptibility was tested by microdilution. Whole-genome sequencing was performed using Illumina MiSeq and Oxford Nanopore technologies, and sequences were examined for macrolide resistance determinants and mobile genetic structures.
Results
Macrolide resistance was detected in 67 H. influenzae (2.6%) and 52 (10.2%) H. parainfluenzae strains and associated with resistance to other antimicrobials (co-trimoxazole, chloramphenicol, tetracycline). Differences in macrolide resistance existed between the two species. Acquired resistance genes were more prevalent in H. parainfluenzae (35/52; 67.3%) than in H. influenzae (12/67; 17.9%). Gene mutations and amino acid substitutions were more common in H. influenzae (57/67; 85%) than in H. parainfluenzae (16/52; 30.8%). Substitutions in L22 and in 23S rRNA were only detected in H. influenzae (34.3% and 29.0%, respectively), while substitutions in L4 and AcrAB/AcrR were observed in both species. The MEGA element was identified in 35 (67.3%) H. parainfluenzae strains, five located in an integrative and conjugative element (ICE); by contrast, 11 (16.4%) H. influenzae strains contained the MEGA element (all in an ICE). A new ICEHpaHUB8 was described in H. parainfluenzae.
Conclusions
Macrolide resistance was higher in H. parainfluenzae than in H. influenzae, with differences in the underlying mechanisms. H. parainfluenzae exhibits co-resistance to other antimicrobials, often leading to an extensively drug-resistant phenotype. This highlights the importance of conducting antimicrobial resistance surveillance.
© The Author(s) 2024. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.
This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/pages/standard-publication-reuse-rights)
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